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KMID : 0624620110440110705
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BMB Reports
2011 Volume.44 No. 11 p.705 ~ p.712
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Recent advances in microfluidic technologies for biochemistry and molecular biology
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Cho Soon-Gwon
Kang Dong-Ku
Choo Jae-Bum
De Mello Andrew J.
Chang Soo-Ik
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Abstract
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Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.
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KEYWORD
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Biosensors, Emulsion PCR, Genomics, High throughput screening, Microfluidics, Protein-protein interaction, Proteomics
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